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Tissue/ cell preparation: Rabbit spleen cells.
In normal adult rabbits, this antibody reacts with 30-60% of peripheral blood leukocytes. The antibody has also been reported to stain: lymphocytes and macrophages in the spleen; lymphocytes in the thymic medulla and some cells in the cortex; Kupffer cells in the liver; and synovial lining cells (i.e., infiltrating macrophages) in inflamed synovium. DQ alpha transcripts have been detected in bone marrow and spleen and, to a lesser extent, in appendix, lymph nodes, and other organs.
Our Abpromise guarantee covers the use of ab25264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.
|IP||Use at an assay dependent concentration.|
ab25264 has not yet been referenced specifically in any publications.