製品の概要

  • 製品名Anti-RIZ1 antibody [33AT1045]
    RIZ1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [33AT1045] to RIZ1
  • 特異性This antibody is specific for RIZ 1. It does not recognize the beta isoform (RIZ 2).
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Fusion protein, corresponding to amino acids 1-347 of Human RIZ1

  • 特記事項


    Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ 1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ 1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ 1 is also a tumor susceptibility gene in mice. The loss of RIZ 1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ 1 promoter strongly correlated with lost or decreased RIZ 1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 189 kDa.
ELISA 1/1000.

ターゲット情報

  • 機能S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
  • 組織特異性Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.
  • 配列類似性Contains 8 C2H2-type zinc fingers.
    Contains 1 SET domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GATA-3-binding protein G3B antibody
    • HUMHOXY1 antibody
    • KMT8 antibody
    • Lysine N-methyltransferase 8 antibody
    • MTB-ZF antibody
    • MTE-binding protein antibody
    • PR domain containing 2, with ZNF domain antibody
    • PR domain zinc finger protein 2 antibody
    • PR domain-containing protein 2 antibody
    • PRDM2 antibody
    • PRDM2_HUMAN antibody
    • Retinoblastoma protein binding zinc finger protein antibody
    • Retinoblastoma protein-interacting zinc finger protein antibody
    • Retinoblastoma protein-interacting zinc-finger protein antibody
    • RIZ antibody
    • RIZ1 antibody
    • RIZ2 antibody
    • Zinc finger DNA binding protein antibody
    • Zinc finger protein RIZ antibody
    see all

Anti-RIZ1 antibody [33AT1045] 画像



  • Predicted band size : 189 kDa


    Western blot analysis of ab3791 in lysate from transformed cells. Lane A: mock plasmid, Lane B: RIZ1[1-347]-encoding plasmid. RIZ1[1-347] (arrow) was detected using ab3791. Secondary HRP-anti-mouse was used for signal visualization with chemiluminescence.

Anti-RIZ1 antibody [33AT1045] (ab3791) 使用論文

This product has been referenced in:
  • Jiang GL & Huang S Adenovirus expressing RIZ1 in tumor suppressor gene therapy of microsatellite-unstable colorectal cancers. Cancer Res 61:1796-8 (2001). Read more (PubMed: 11280725) »
  • Liu L  et al. The retinoblastoma interacting zinc finger gene RIZ produces a PR domain-lacking product through an internal promoter. J Biol Chem 272:2984-91 (1997). Read more (PubMed: 9006946) »

See all 2 Publications for this product

Product Wall

Thank you for submitting an Abreview of ab3791. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this ...

Read More
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 60 µg
Specification Mouse ES Cells
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Utkarsh Kapoor

Verified customer

投稿 Dec 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"