Anti-RIPX 抗体 (ab89147)
Key features and details
- Mouse polyclonal to RIPX
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RIPX antibody
RIPX 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to RIPX -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length protein within Human RIPX. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Rat brain tissue lysate and RIPX transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. |
ターゲット情報
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機能
Implicated in the formation of a single axon by developing neurons. May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes. -
配列類似性
Contains 1 RUN domain. -
翻訳後修飾
Isoform 1 is partially phosphorylated. -
細胞内局在
Cell projection. Enriched in F-actin enriched filopodia and lamellipodia of growth cones of axons and minor processes of hippocampal neurons in culture. - Information by UniProt
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参照データベース
- Entrez Gene: 22902 Human
- Entrez Gene: 360921 Rat
- GenBank: NM_014961 Human
- Omim: 611194 Human
- SwissProt: Q7L099 Human
- SwissProt: Q5FVJ0 Rat
- Unigene: 740904 Human
- Unigene: 228806 Rat
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別名
- Protein RUFY3 antibody
- rap2 interacting protein x antibody
- Rap2 interacting protein x isoform 2 antibody
see all
画像
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Anti-RIPX antibody (ab89147) at 1 µg/ml + Rat brain tissue lysate at 50 µg
Predicted band size: 53 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted? -
All lanes : Anti-RIPX antibody (ab89147) at 1 µg/ml
Lane 1 : RIPX transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab89147 は 2 報の論文で使用されています。
- Men W et al. RUFY3 Predicts Poor Prognosis and Promotes Metastasis through Epithelial-mesenchymal Transition in Lung Adenocarcinoma. J Cancer 10:6278-6285 (2019). PubMed: 31772661
- Xie R et al. RUFY3 interaction with FOXK1 promotes invasion and metastasis in colorectal cancer. Sci Rep 7:3709 (2017). PubMed: 28623323