Anti-RIP3 抗体 [Rippy-3] (ab16090)

製品の概要

  • 製品名Anti-RIP3 antibody [Rippy-3]
    RIP3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Rippy-3] to RIP3
  • 特異性The antibody has been tested on HEK 293T cells transfected with an expression plasmid coding for human RIP3. It has not been tested against the endogenous protein.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein corresponding to Human RIP3.
    Database link: Q9Y573

  • ポジティブ・コントロール
    • recombinant protein

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.

ターゲット情報

  • 機能Promotes apoptosis.
  • 組織特異性Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

Anti-RIP3 antibody [Rippy-3] 画像

  • All lanes : Anti-RIP3 antibody [Rippy-3] (ab16090) at 1/1000 dilution

    Lane 1 : HEK 293T cells(5x105) mock transfected
    Lane 2 : HEK 293T cells (5x105)transfected with an expression plasmid coding for human RIP3


    Predicted band size : 62 kDa

Anti-RIP3 antibody [Rippy-3] (ab16090) 使用論文

ab16090 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. We do have positive controls available for these antibodies. The Jurkat lysate catalog number is ab7899. This should also be an appropriate control for the anti-RIP3 antibody, as we do not have the HEK293 transfected cells ...

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Merci pour votre patience.

Notre recherche interne donnais comme seul candidat l'anticorpsab72106 qui pourrait avoir été utilisé dans la publication que vous nous avez cité. Malheureusement est il très peu p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF cells)
Loading amount 15 µg
Specification MEF cells
Treatment hydroxyurea for 1h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Mar 26 2007

Thank you for providing the customer's results and protocol, it is very clear that there is a problem with the antibody and I will investigate this matter further and will consider removing this antibody from our catalogue. I have asked our accounts...

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Thank you for providing so much information about the Western Blotting protocol used with this antibody. The researcher has clearly tried many steps to optimise the experimental conditions, but there's one thing they have not yet tried which I think wi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"