Anti-RIP3 抗体 (ab62344)
Key features and details
- Rabbit polyclonal to RIP3
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-RIP3 antibody
RIP3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RIP3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Mouse RIP3 (C terminal).
Database link: Q9Z2P5 -
ポジティブ・コントロール
- WB: Mouse C2C12 cell lysate. IHC-P: Mouse kidney tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62344の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Primary antibody incubation: 1h incubation at Room Temperature in 5% NFDM/TBST. Observed: 53kD for mouse RIP3 |
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IHC-P | (1) |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). Primary antibody incubation: 1h incubation at Room Temperature in 5% NFDM/TBST. Observed: 53kD for mouse RIP3 |
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Promotes apoptosis. -
組織特異性
Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 56532 Mouse
- SwissProt: Q9QZL0 Mouse
- Unigene: 46612 Mouse
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別名
- Receptor interacting protein 3 antibody
- Receptor interacting serine threonine kinase 3 antibody
- Receptor interacting serine/threonine protein kinase 3 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with ab62344 at 2.5 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Lanes 1-2 : Anti-RIP3 antibody (ab62344) at 0.1 µg/ml
Lane 3 : Anti-RIP3 antibody (ab62344) at 0.2 µg/ml
Lane 4 : Anti-RIP3 antibody (ab62344) at 0.5 µg/ml
Lane 1 : C2C12 (mouse myoblast cell line) cell lysate with Peptide blocking
Lanes 2-4 : C2C12 (mouse myoblast cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 57 kDaPrimary antibody incubation: 1h incubation at Room Temperature in 5% NFDM/TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (40)
ab62344 は 40 報の論文で使用されています。
- Piao SG et al. Inhibition of RIP1-RIP3-mediated necroptosis attenuates renal fibrosis via Wnt3α/β-catenin/GSK-3β signaling in unilateral ureteral obstruction. PLoS One 17:e0274116 (2022). PubMed: 36223414
- Li Y et al. Generative deep learning enables the discovery of a potent and selective RIPK1 inhibitor. Nat Commun 13:6891 (2022). PubMed: 36371441
- Huang Y et al. Autophagy-Related LC3 Accumulation Interacted Directly With LIR Containing RIPK1 and RIPK3, Stimulating Necroptosis in Hypoxic Cardiomyocytes. Front Cell Dev Biol 9:679637 (2021). PubMed: 34368130
- Fang P et al. RIP3-mediated necroptosis increases neuropathic pain via microglia activation: necrostatin-1 has therapeutic potential. FEBS Open Bio 11:2858-2865 (2021). PubMed: 34320280
- Wu Y et al. RIP1/RIP3/MLKL Mediates Myocardial Function Through Necroptosis in Experimental Autoimmune Myocarditis. Front Cardiovasc Med 8:696362 (2021). PubMed: 34497836