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Our Abpromise guarantee covers the use of ab57954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: RIP2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Ramos cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab57954 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab57954 was shown to recognize RIP2 when RIP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab57954 and ab181602 (loading control to GAPDH) were diluted 1 µg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.