製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to RIP140
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-EKDSPRLTKTNP

    , corresponding to internal sequence amino acids 1055-1066 of Human RIP140 (NP_003480.2).

  • ポジティブ・コントロール
    • Human Cerebellum lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 129 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors.
    • ドメイン
      Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding.
    • 翻訳後修飾
      Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH.
    • 細胞内局在
      Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1.
    • Information by UniProt
    • 参照データベース
    • 別名
      • NRIP 1 antibody
      • NRIP1 antibody
      • NRIP1_HUMAN antibody
      • Nuclear factor RIP 140 antibody
      • Nuclear factor RIP140 antibody
      • Nuclear receptor interacting protein 1 antibody
      • Nuclear receptor-interacting protein 1 antibody
      • Receptor interacting protein 140 antibody
      • Receptor-interacting protein 140 antibody
      • RIP 140 antibody
      • RIP140 antibody
      see all

    画像

    • Anti-RIP140 antibody (ab91476) at 0.5 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 129 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab91476 has not yet been referenced specifically in any publications.

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab91476.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録