Anti-RIP140 抗体 (ab91476)
Key features and details
- Goat polyclonal to RIP140
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RIP140 antibody
RIP140 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RIP140 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog -
免疫原
Synthetic peptide:
C-EKDSPRLTKTNP
, corresponding to internal sequence amino acids 1055-1066 of Human RIP140 (NP_003480.2). -
ポジティブ・コントロール
- Human Cerebellum lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab91476 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab91476の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 129 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 129 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. -
ドメイン
Contains 9 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs, which have different affinities for nuclear receptors. The C-terminal LTKTNPILYYMLQK motif is required for ligand-dependent interaction with RAAR and RXRB homodimers and heterodimers, for the corepressor activity, and for the formation of an HDAC3 complex with RARA/RXRB (By similarity). Contains at least four autonomous repression domains (RD1-4). RD1 functions via a histone deacetylase (HDAC)-independent mechanism, whereas RD2, RD3 and RD4 can function by HDAC-dependent or independent mechanisms, depending on cell type. RD2 is dependent on CTBP binding. -
翻訳後修飾
Acetylation regulates its nuclear translocation and corepressive activity (By similarity). Acetylation abolishes interaction with CTBP1. Phosphorylation enhances interaction with YWHAH. -
細胞内局在
Nucleus. Localized to discrete foci and redistributes to larger nuclear domains upon binding to ligand-bound NR3C1. - Information by UniProt
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参照データベース
- Entrez Gene: 524960 Cow
- Entrez Gene: 478385 Dog
- Entrez Gene: 8204 Human
- Entrez Gene: 268903 Mouse
- Entrez Gene: 304157 Rat
- Omim: 602490 Human
- SwissProt: P48552 Human
- SwissProt: Q8CBD1 Mouse
see all -
別名
- NRIP 1 antibody
- NRIP1 antibody
- NRIP1_HUMAN antibody
see all
画像
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Anti-RIP140 antibody (ab91476) at 0.5 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 129 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (2)
ab91476 は 2 報の論文で使用されています。
- Huang M et al. NRIP1 aggravates lung injury caused by Pseudomonas aeruginosa in mice by increasing PIAS1 ubiquitination. Aging (Albany NY) 14:3529-3539 (2022). PubMed: 35460552
- Zhao L et al. COX7A1 suppresses the viability of human non-small cell lung cancer cells via regulating autophagy. Cancer Med 8:7762-7773 (2019). PubMed: 31663688