Anti-RING2 / RING1B / RNF2 抗体 - ChIP Grade (ab3832)

製品の概要

  • 製品名
    Anti-RING2 / RING1B / RNF2 antibody - ChIP Grade
    RING2 / RING1B / RNF2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to RING2 / RING1B / RNF2 - ChIP Grade
  • 特異性
    This antibody recognises a band at just above the expected size of 37kD.
  • アプリケーション
    適用あり: ICC/IF, WB, ChIPmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide:

    EAGPSNKRTKTSDC

    , corresponding to amino acids 189-201 of Human RING1B.

  • 特記事項

    RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/300. (see Abreviews)
WB Use at an assay dependent dilution. Detects a band of approximately 43 kDa (predicted molecular weight: 37.6 kDa).
ChIP Use at an assay dependent dilution.

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 RING-type zinc finger.
  • 翻訳後修飾
    Polyubiquitinated in the presence of UBE2D3 (in vitro).
    Monoubiquitinated, by auto-ubiquitination.
  • 細胞内局在
    Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BAP 1 antibody
    • BAP1 antibody
    • DING antibody
    • DinG protein antibody
    • E3 ubiquitin protein ligase RING 2 antibody
    • E3 ubiquitin protein ligase RING2 antibody
    • E3 ubiquitin-protein ligase RING2 antibody
    • HIP2 interacting protein 3 antibody
    • HIP2-interacting protein 3 antibody
    • HIPI 3 antibody
    • HIPI3 antibody
    • Huntingtin interacting protein 2 interacting protein 3 antibody
    • Huntingtin-interacting protein 2-interacting protein 3 antibody
    • OTTHUMP00000060668 antibody
    • Polycomb M33 interacting protein Ring 1B antibody
    • Polycomb M33 interacting protein Ring1B antibody
    • Protein DinG antibody
    • RING 1B antibody
    • RING 2 antibody
    • RING finger protein 1B antibody
    • RING finger protein 2 antibody
    • RING finger protein BAP 1 antibody
    • RING finger protein BAP-1 antibody
    • RING finger protein BAP1 antibody
    • RING1b antibody
    • RING2_HUMAN antibody
    • RNF 2 antibody
    • Rnf2 antibody
    see all

画像



  • Predicted band size : 37.6 kDa

    Western blot using ab3832 at 1/500. Lysates at 20µg/lane.

    Lane 1: 3T3 cell lysate
    Lane 2: U937 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Mouse brain lysate
    Lane 5: CHO-K1 cell lysate

    Secondary ab: rabbit polyclonal to goat IgG ab6741 1/5000
    Exposure time: 3 minutes

    Western blot using ab3832 at 1/500. Lysates at 20µg/lane.

    Lane 2: U937 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Mouse brain lysate
    Lane 5: CHO-K1 cell lysate

    Secondary ab: rabbit polyclonal to goat IgG ab6741 1/5000 Exposure time: 3 minutes
  • ab3832 staining RING2/RING1B/RNF2 in Human Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 0.2% fish scale gelatin) for 20 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-goat IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:
  • Qu C & Qu Y Down-regulation of salt-inducible kinase 1 (SIK1) is mediated by RNF2 in hepatocarcinogenesis. Oncotarget 8:3144-3155 (2017). WB ; Human . Read more (PubMed: 27911266) »
  • Toth Z  et al. LANA-Mediated Recruitment of Host Polycomb Repressive Complexes onto the KSHV Genome during De Novo Infection. PLoS Pathog 12:e1005878 (2016). Human . Read more (PubMed: 27606464) »

See all 15 Publications for this product

レビューと Q&A

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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Thank you for contacting us.
I am sorry to hear the antibody does not give you results as expected.

I do have a couple questions in order to understand your experiment better:

1) Are you using the antibody for IP or ChIP? The...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cells)
Specification
Hela cells
Fixative
Methanol
Permeabilization
No
Blocking step
Fish scale gelatin in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Loading amount
100000 cells
Specification
Jurkat cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 17 2010

Thank you for your enquiry. I have consulted the EXPASY databases and I retrieved the following information: Mel18 - Tissue specificity: Detected in all tissues examined with high expression found in placenta, lung and kidney and low expression, in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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