製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).

ターゲット情報

  • 機能E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 RING-type zinc finger.
  • 翻訳後修飾Polyubiquitinated in the presence of UBE2D3 (in vitro).
    Monoubiquitinated, by auto-ubiquitination.
  • 細胞内局在Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BAP 1 antibody
    • BAP1 antibody
    • DING antibody
    • DinG protein antibody
    • E3 ubiquitin protein ligase RING 2 antibody
    • E3 ubiquitin protein ligase RING2 antibody
    • E3 ubiquitin-protein ligase RING2 antibody
    • HIP2 interacting protein 3 antibody
    • HIP2-interacting protein 3 antibody
    • HIPI 3 antibody
    • HIPI3 antibody
    • Huntingtin interacting protein 2 interacting protein 3 antibody
    • Huntingtin-interacting protein 2-interacting protein 3 antibody
    • OTTHUMP00000060668 antibody
    • Polycomb M33 interacting protein Ring 1B antibody
    • Polycomb M33 interacting protein Ring1B antibody
    • Protein DinG antibody
    • RING 1B antibody
    • RING 2 antibody
    • RING finger protein 1B antibody
    • RING finger protein 2 antibody
    • RING finger protein BAP 1 antibody
    • RING finger protein BAP-1 antibody
    • RING finger protein BAP1 antibody
    • RING1b antibody
    • RING2_HUMAN antibody
    • RNF 2 antibody
    • Rnf2 antibody
    see all

Anti-RING2 / RING1B / RNF2 antibody 画像

  • Anti-RING2 / RING1B / RNF2 antibody (ab14751) at 0.5 µg/ml + Cell lysate prepared from human K562 cells at 35 µg
    Developed using the ECL technique

    Predicted band size : 38 kDa

Anti-RING2 / RING1B / RNF2 antibody (ab14751) 使用論文

This product has been referenced in:
  • Zhang Y  et al. Ubiquitination and degradation of ribonucleotide reductase M1 by the polycomb group proteins RNF2 and Bmi1 and cellular response to gemcitabine. PLoS One 9:e91186 (2014). Human . Read more (PubMed: 24614341) »
  • Charan RA  et al. Inhibition of apoptotic Bax translocation to the mitochondria is a central function of parkin. Cell Death Dis 5:e1313 (2014). Read more (PubMed: 24991765) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"