Anti-RING2 / RING1B / RNF2 抗体 (ab101274)


  • 製品名
    Anti-RING2 / RING1B / RNF2 antibody
    RING2 / RING1B / RNF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RING2 / RING1B / RNF2
  • 由来種
  • アプリケーション
    適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residue 286 and 336 of Human RING2/ RING1B/ RNF2 (NP_009143.1).

  • ポジティブ・コントロール
    • K562 whole cell lysate


  • 製品の状態
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
  • アイソタイプ
  • 研究分野


Our Abpromise guarantee covers the use of ab101274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Contains 1 RING-type zinc finger.
    • 翻訳後修飾
      Polyubiquitinated in the presence of UBE2D3 (in vitro).
      Monoubiquitinated, by auto-ubiquitination.
    • 細胞内局在
      Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BAP 1 antibody
      • BAP1 antibody
      • DING antibody
      • DinG protein antibody
      • E3 ubiquitin protein ligase RING 2 antibody
      • E3 ubiquitin protein ligase RING2 antibody
      • E3 ubiquitin-protein ligase RING2 antibody
      • HIP2 interacting protein 3 antibody
      • HIP2-interacting protein 3 antibody
      • HIPI 3 antibody
      • HIPI3 antibody
      • Huntingtin interacting protein 2 interacting protein 3 antibody
      • Huntingtin-interacting protein 2-interacting protein 3 antibody
      • OTTHUMP00000060668 antibody
      • Polycomb M33 interacting protein Ring 1B antibody
      • Polycomb M33 interacting protein Ring1B antibody
      • Protein DinG antibody
      • RING 1B antibody
      • RING 2 antibody
      • RING finger protein 1B antibody
      • RING finger protein 2 antibody
      • RING finger protein BAP 1 antibody
      • RING finger protein BAP-1 antibody
      • RING finger protein BAP1 antibody
      • RING1b antibody
      • RING2_HUMAN antibody
      • RNF 2 antibody
      • Rnf2 antibody
      see all


    • Detection of RING2 / RING1B / RNF2 in Immunoprecipitates of K562 whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101274 at 3 µg/mg lysate for IP. An anti-RING2 / RING1B / RNF2 antibody which recognizes an upstream epitope was used at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.



    ab101274 has not yet been referenced specifically in any publications.

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