This antibody gave a positive control in the following human lysates:
This antibody gave a positive control in the following mouse lysate:
This antibody gave a positive control in the following rat lysate:
Kidney Whole Cell Lysate - normal tissue
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 20237320
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 42,39 kDa).
機能Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
パスウェイProtein modification; protein ubiquitination.
Basu A et al. YY1 DNA binding and interaction with YAF2 is essential for Polycomb recruitment. Nucleic Acids ResN/A:N/A (2013).
Read more (PubMed: 24285299) »
Argiropoulos B et al. Linkage of the potent leukemogenic activity of Meis1 to cell-cycle entry and transcriptional regulation of cyclin D3. Blood115:4071-82 (2010).
Read more (PubMed: 20237320) »