製品の概要

  • 製品名Anti-RICTOR antibody [7B3]
    RICTOR 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7B3] to RICTOR
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Human, Monkey
  • 免疫原

    Purified recombinant fragment of Human RICTOR expressed in E. Coli.

  • ポジティブ・コントロール
    • HeLa, PANC-1, MOLT4, HepG2 and HEK293 cell lysates. Thyroid gland tissues and Human breast carcinoma. NIH3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 192 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.

ターゲット情報

  • 機能Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
  • 配列類似性Belongs to the RICTOR family.
  • 翻訳後修飾Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AVO3 antibody
    • AVO3 homolog antibody
    • DKFZp686B11164 antibody
    • hAVO3 antibody
    • KIAA1999 antibody
    • Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae) antibody
    • mAVO3 antibody
    • MGC39830 antibody
    • PIA antibody
    • Pianissimo antibody
    • Rapamycin insensitive companion of mTOR antibody
    • Rapamycin-insensitive companion of mTOR antibody
    • Rictor antibody
    • RICTR antibody
    • RICTR_HUMAN antibody
    • RPTOR independent companion of MTOR complex 2 antibody
    • TORC2 specific protein AVO3 antibody
    see all

Anti-RICTOR antibody [7B3] 画像

  • All lanes : Anti-RICTOR antibody [7B3] (ab104838) at 1/500 dilution

    Lane 1 : HeLa cell lysate.
    Lane 2 : PANC-1 cell lysate.
    Lane 3 : MOLT4 cell lysate.
    Lane 4 : HepG2 cell lysate.
    Lane 5 : HEK293 cell lysate.


    Predicted band size : 192 kDa
  • ab104838 at 1/200 dilution staining RICTOR in NIH3T3 cells (green) by immunofluorescence. DRAQ5 fluorescent DNA dye is shown in blue. Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • ab104838 at 1/200 dilution staining RICTOR in paraffin-embedded thyroid gland tissues by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab104838 at 1/200 dilution staining RICTOR in paraffin-fixed Human breast carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab104838 used for Flow cytometric analysis of HeLa cells (green). The negative control is shown in purple.

Anti-RICTOR antibody [7B3] (ab104838) 使用論文

This product has been referenced in:
  • Hütt-Cabezas M  et al. Activation of mTORC1/mTORC2 signaling in pediatric low-grade glioma and pilocytic astrocytoma reveals mTOR as a therapeutic target. Neuro Oncol 15:1604-14 (2013). IHC-P ; Human . Read more (PubMed: 24203892) »

See 1 Publication for this product

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