Anti-RIC8A 抗体 (ab97808)
Key features and details
- Rabbit polyclonal to RIC8A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RIC8A antibody
RIC8A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RIC8A -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to a region within amino acids 528 and 537 of Human RIC8A (NP_068751).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97808の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
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特記事項 |
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IHC-P
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 60 kDa. |
ターゲット情報
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機能
Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation. -
配列類似性
Belongs to the synembryn family. -
細胞内局在
Cytoplasm. Cell membrane. Colocalizes with RIC8A in CA2 hippocampal neurons. Colocalizes with GNAI1 and RGS14 at the plasma membrane (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 60626 Human
- Omim: 609146 Human
- SwissProt: Q9NPQ8 Human
- Unigene: 592292 Human
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別名
- MGC104517 antibody
- MGC131931 antibody
- MGC148073 antibody
see all
画像
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All lanes : Anti-RIC8A antibody (ab97808) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 60 kDa
7.5% SDS PAGE -
ab97808, at a 1/100 dilution, staining RIC8A in paraffin embedded Adrenal cortical tumor by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab97808 は 3 報の論文で使用されています。
- Zeng H et al. Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC. Elife 8:N/A (2019). PubMed: 31741433
- Chishiki K et al. Ric-8A, an activator protein of Gai, controls mammalian epithelial cell polarity for tight junction assembly and cystogenesis. Genes Cells 22:293-309 (2017). PubMed: 28185378
- Boularan C et al. Resistance to inhibitors of cholinesterase (Ric)-8A and Gai contribute to cytokinesis abscission by controlling vacuolar protein-sorting (Vps)34 activity. PLoS One 9:e86680 (2014). WB, IP, ICC/IF ; Human . PubMed: 24466196