製品の概要

  • 製品名Anti-ribosomal RNA 5.8s antibody [Y10b]
    ribosomal RNA 5.8s 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Y10b] to ribosomal RNA 5.8s
  • 特異性This antibody is specific for ribosomal RNA. There is no cross-reactivity with other RNAs.
  • アプリケーション適用あり: IP, ICC/IFmore details
  • 種交差性
    This antibody reacts with all eukaryotes.
  • 免疫原

    The whole 5.8S ribosomal RNA.

  • エピトープThis antibody is useful for recognizing RNA epitopes on both the large and small subunit.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Protein A purified mouse ascites.
  • ポリ/モノモノクローナル
  • クローン名Y10b
  • アイソタイプIgG3
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 関連性The inhibition of protein synthesis by specific anti 5.8S rRNA oligonucleotides have suggested that this RNA plays an important role in eukaryotic ribosome function, specifically ribosomal translocation. Mutations in the 5.8S rRNA can inhibit cell growth and compromise protein synthesis in vitro.
  • 細胞内局在Cytoplasmic and Nuclear

Anti-ribosomal RNA 5.8s antibody [Y10b] (ab37144) 使用論文

This product has been referenced in:
  • Zhu CH  et al. SGNP: an essential Stress Granule/Nucleolar Protein potentially involved in 5.8s rRNA processing/transport. PLoS ONE 3:e3716 (2008). ICC/IF ; Human . Read more (PubMed: 19005571) »
  • Thomas MG  et al. Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes. Mol Biol Cell 16:405-20 (2005). Read more (PubMed: 15525674) »

See all 14 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Lymphoblast)
Specification Lymphoblast
Permeabilization Yes - 0.1% TX-100 for 10 min.
Fixative Paraformaldehyde
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投稿 Mar 26 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (brain)
Specification brain
Fixative paraformaldehyde + glutaraldehyde
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 22°C
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投稿 Dec 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"