Anti-Ribosomal protein S10 抗体 (ab95944)

製品の概要

  • 製品名
    Anti-Ribosomal protein S10 antibody
    Ribosomal protein S10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ribosomal protein S10
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Zebrafish
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 - 161 of Human Ribosomal protein S10 (NP_001005)

  • ポジティブ・コントロール
    • 293T, A431, H1299, HepG2, MOLT4 and Raji cell lysates IF: HeLa cells IHC-P: A549 xenograft

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 19 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Component of the 40S ribosomal subunit.
  • 関連疾患
    Defects in RPS10 are the cause of Diamond-Blackfan anemia type 9 (DBA9) [MIM:613308]. It is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre- Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • 配列類似性
    Belongs to the ribosomal protein S10e family.
  • 翻訳後修飾
    Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation.
  • 細胞内局在
    Cytoplasm. Nucleus > nucleolus. Localized in the granular component (GC) region of the nucleolus. Methylation is required for its localization in the GC region. Co-localizes with NPS1 in the GC region of the nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 40S ribosomal protein S10 antibody
    • DBA9 antibody
    • MGC88819 antibody
    • OTTHUMP00000016229 antibody
    • OTTHUMP00000016230 antibody
    • RPS10 antibody
    • RS10_HUMAN antibody
    • S10 antibody
    see all

画像

  • Anti-Ribosomal protein S10 antibody (ab95944) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 19 kDa
  • ab95944, at a 1/200 dilution, staining Ribosomal protein S10 in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
  • ab95944, at a 1/500 dilution, staining of Ribosomal protein S10 in a paraffin-embedded A549 xenograft by Immunohistochemical analysis.

参考文献

ab95944 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
PBS-1% ovalbumin as blocking agent for 20 minute(s) · Concentration: 1.0% · Temperature: 22°C
Sample
Salmo salar Cell (testicular)
Specification
testicular
Permeabilization
Yes - methyl methacrylate/Butyl methacrylate
Fixative
glutaraldehyde-formaldehyde
Username

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投稿 Mar 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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