製品の概要

  • 製品名
    Anti-Ribophorin I antibody
    Ribophorin I 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Ribophorin I
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 629-728 of Saccharomyces cerevisiae Ribophorin I. [Swiss Prot P38764] Target sequence used to make the antibody: VKGEEDADEE ETAEGQTNSI SDFLGEQVNE PTKNEEAEIE VDEMEVDAEG EEVEVKAEIT EKKNGESLEG EEIKSEEKKG KSSDKDATTD GKNDDEEEEK.

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度
    Whole antiserum
  • 一次抗体 備考
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 109 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能
    Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
  • 組織特異性
    Expressed in all tissues tested.
  • 配列類似性
    Belongs to the OST1 family.
  • 細胞内局在
    Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 別名
    • D6Wsu137e antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit antibody
    • Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit antibody
    • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 antibody
    • EC 2.4.1.119 antibody
    • Oligosaccharyltransferase 1 homolog antibody
    • oligosaccharyltransferase complex subunit (non-catalytic) antibody
    • OST1 antibody
    • RBPH1 antibody
    • Ribophorin I antibody
    • Ribophorin-1 antibody
    • RPN-I antibody
    • Rpn1 antibody
    • RPN1_HUMAN antibody
    see all

プロトコール

参考文献

ab52672 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Pichia pastoris Cell lysate - other (membrane fraction (sucrose gradient))
Loading amount
2 µg
Specification
membrane fraction (sucrose gradient)
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Laura Baciou

Verified customer

投稿 Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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