Anti-Ribonuclease A 抗体 (ab94417)

製品の概要

  • 製品名Anti-Ribonuclease A antibody
    Ribonuclease A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ribonuclease A
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Pig, Chimpanzee, Drosophila melanogaster, African green monkey, Spider monkey
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (HITDCRLTNG SRYPNCAYRT SPKERHIIVA CEGSPYVPVH FDASVEDST) of Human Ribonuclease A (NP_002924).

  • ポジティブ・コントロール
    • Human fetal kidney lysate IHC-P: Human lung FFPE tissue sections.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab94417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-Ribonuclease A antibody 画像

  • IHC image of Ribonuclease A staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94417, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Ribonuclease A antibody (ab94417) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal kidney lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 18 kDa
    Gel concentration: 10-20%

Anti-Ribonuclease A antibody (ab94417) 使用論文

ab94417 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Bos taurus Purified protein (mixture of RNase A and B from pancreas)
Gel Running Conditions Reduced Denaturing (10-20)
Loading amount 20 µg
Treatment PNGase treatment for 1 hour at 37C
Specification mixture of RNase A and B from pancreas
Blocking step ECL prime blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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投稿 Feb 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"