製品の概要

  • 製品名Anti-Ribonuclease 3 antibody
    Ribonuclease 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit Polyclonal to Ribonuclease 3
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 123-151 of Human Ribonuclease 3 (NP_002926.2).

  • ポジティブ・コントロール
    • NCI-H460 cell line lysates This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab116017 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG

アプリケーション

Our Abpromise guarantee covers the use of ab116017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity.
  • 配列類似性Belongs to the pancreatic ribonuclease family.
  • 細胞内局在Cytoplasmic granule. Matrix of eosinophil's large specific granule.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cytotoxic ribonuclease antibody
    • ECP antibody
    • ECP_HUMAN antibody
    • Eosinophil cationic protein antibody
    • OTTHUMP00000164017 antibody
    • Ribonuclease 3 antibody
    • Ribonuclease, RNase A family, 3 antibody
    • RNase 3 antibody
    • RNASE3 antibody
    • RNS3 antibody
    see all

Anti-Ribonuclease 3 antibody 画像

  • IHC image of Ribonuclease 3 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116017, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Ribonuclease 3 antibody (ab116017) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 18 kDa

Anti-Ribonuclease 3 antibody (ab116017) 使用論文

ab116017 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab116017.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"