Rabbit monoclonal [EPR2806] to RIAM
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human RIAM aa 1-100 (N terminal).
Human Lymph node, Fetal brain, Human spleen, Jurkat and HL-60 + retinoic acid cell lysates; Human tonsil tissue
This product is a recombinant rabbit monoclonal antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RIAM (Rap1-GTP interacting adapter molecule) appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling via interaction with profilin and VASP. It suppresses insulin-induced promoter activities through AP1 and SRE and also mediates Rap1-induced adhesion.
Cell Membrane, Cytoplasm and cytoskeleton
Amyloid beta A4 precursor protein binding family B member 1 interacting protein antibody
Amyloid beta precursor protein binding family B member 1 antibody
Western blot - RIAM antibody [EP2806] (ab92537)
All lanes : Anti-RIAM antibody [EPR2806] (ab92537) at 1/2000 dilution Lane 1 : Human Lymph node lysate Lane 2 : Fetal brain lysate Lane 3 : Human spleen lysate Lane 4 : Jurkat cell lysate Lane 5 : HL-60 + retinoic acid cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 73 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RIAM antibody [EP2806] (ab92537)
ab92537, at 1/100 dilution, staining RIAM in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"