Anti-Rhp51 抗体 (ab63799)
Key features and details
- Rabbit polyclonal to Rhp51
- Suitable for: WB, IP, ICC/IF
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
製品の概要
-
製品名
Anti-Rhp51 antibody -
製品の詳細
Rabbit polyclonal to Rhp51 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IFmore details -
種交差性
交差種: Schizosaccharomyces pombe -
免疫原
Purified recombinant full length protein (Schizosaccharomyces pombe)
-
ポジティブ・コントロール
- Schizosaccharomyces pombe whole cell extracts.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading...
-
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63799の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 40 kDa).
|
|
IP |
Use at an assay dependent concentration.
|
|
ICC/IF |
1/500.
|
特記事項 |
---|
WB
1/2000 - 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 40 kDa). |
IP
Use at an assay dependent concentration. |
ICC/IF
1/500. |
ターゲット情報
-
関連性
Rhp51 is required both for recombination and for the repair of DNA damage caused by X-rays. Binds to single and double stranded DNA, in the presence of magnesium, and exhibits DNA-dependent ATPase activity. Promotes DNA strand annealing and strand exchange via DNA recombinase activity and forms helical nucleoprotein filaments. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 2543580 Schizosaccharomyces pombe
- SwissProt: P36601 Schizosaccharomyces pombe
-
別名
- DNA repair protein rhp51 antibody
- Rad51 antibody
- RAD51 homolog antibody
- Recombinase Rhp51 antibody
画像
-
ab63799 (1/500) staining Rhp51 in S. pombe cells without or with MMS (0.025%) treatment for 1 hour by ICC/IF (Immunocytochemistry/immunofluorescence).
-
All lanes : Anti-Rhp51 antibody (ab63799) at 1/2000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Whole cell extract of Schizosaccharomyces pombe wild-type strain
Lane 3 : Whole cell extract of Schizosaccharomyces pombe Rad51 deletion strain
Predicted band size: 40 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab63799 は 3 報の論文で使用されています。
- Muraszko J et al. Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast. Nucleic Acids Res 49:6832-6848 (2021). PubMed: 34157114
- Kramarz K et al. The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal. Nat Commun 11:5643 (2020). PubMed: 33159083
- Matmati S et al. Telomerase Repairs Collapsed Replication Forks at Telomeres. Cell Rep 30:3312-3322.e3 (2020). PubMed: 32160539