製品の概要

  • 製品名Anti-RHOJ antibody
    RHOJ 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RHOJ
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 71 - 120 (LGLYDTAGQE DYNQLRPLSY PNTDVFLICF SVVNPASYHN VQEEWVPELK) of Human RHOJ (NP_065714).

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab105311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology.
  • 配列類似性Belongs to the small GTPase superfamily. Rho family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARHJ antibody
    • Ras homolog family member J antibody
    • Ras homolog gene family member J antibody
    • RAS like family 7 member B antibody
    • Ras like protein family member 7B antibody
    • Ras-like protein family member 7B antibody
    • RASL 7B antibody
    • RASL7B antibody
    • Rho related GTP binding protein RhoJ antibody
    • Rho-related GTP-binding protein RhoJ antibody
    • RHOI antibody
    • Rhoj antibody
    • RHOJ_HUMAN antibody
    • Tc10 like GTP binding protein TCL antibody
    • TC10 like Rho GTPase antibody
    • Tc10-like GTP-binding protein antibody
    • TC10B antibody
    • TCL antibody
    see all

Anti-RHOJ antibody 画像

  • Anti-RHOJ antibody (ab105311) at 1 µg/ml + 721 B cell lysate at 10 µg

    Predicted band size : 24 kDa

Anti-RHOJ antibody (ab105311) 使用論文

ab105311 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse leukemic stem cells)
Gel Running Conditions Reduced Non-Denaturing (Native) (4- 20%)
Loading amount 40 µg
Specification Mouse leukemic stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 19 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hek293 cells transfected with RhoJ-GFP)
Loading amount 20 µg
Specification Hek293 cells transfected with RhoJ-GFP
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jul 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"