製品の概要

  • 製品名Anti-RHOH antibody
    RHOH 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RHOH
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide KLH conjugated corresponding to a region from the C terminus of Human RHOH

  • ポジティブ・コントロール
    • Human colon and small intestine tissue. HT-29 cell lysate and A549 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca(2+) mobilization in mast cells (By similarity). Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils.
  • 組織特異性Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis.
  • 関連疾患Note=A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6.
  • 配列類似性Belongs to the small GTPase superfamily. Rho family.
  • ドメインThe region involved in interaction with ZAP70 is a non-canonical immunoreceptor tyrosine-based activation motif (ITAM).
  • 翻訳後修飾Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development.
  • 細胞内局在Cytoplasm. Cell membrane. Co-localizes together with ZAP70 in the immunological synapse.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARHH antibody
    • GTP binding protein TTF antibody
    • GTP-binding protein TTF antibody
    • OTTHUMP00000125213 antibody
    • Ras homolog gene family member H antibody
    • Rho related GTP binding protein antibody
    • Rho related GTP binding protein RhoH antibody
    • Rho-related GTP-binding protein RhoH antibody
    • RHOH antibody
    • RHOH_HUMAN antibody
    • Translocation three four protein antibody
    • TTF antibody
    • TTF translocation three four antibody
    see all

Anti-RHOH antibody 画像

  • ab118507 at 5 µg/ml staining RHOH in Formalin-Fixed, Paraffin-Embedded Human colon tissue.
  • ab118507 at 5 µg/ml staining RHOH in Formalin-Fixed, Paraffin-Embedded Human Small Intestine tissue.
  • All lanes : Anti-RHOH antibody (ab118507) at 1/500 dilution

    Lane 1 : HT-29 extract without the synthesized peptide.
    Lane 2 : HT-29 extract with the synthesized peptide.


    Predicted band size : 21 kDa
  • Immunofluorescence analysis of A549 cells, using ab118507 at 1/100. The picture on the right is treated with the synthesized peptide.

Anti-RHOH antibody (ab118507) 使用論文

ab118507 has not yet been referenced specifically in any publications.

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