製品の概要

  • 製品名
    Anti-RhoGDI (phospho S174) antibody
    RhoGDI 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RhoGDI (phospho S174)
  • 特異性
    The antibody detects endogenous levels of RhoGDI only when phosphorylated at serine 174.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized phosphopeptide derived from human RhoGDI around the phosphorylation site of serine 174 (R-G-SP-Y-S).

  • ポジティブ・コントロール
    • IHC-P: Human cervix carcinoma tissue. WB: HUVEC cells treated with EGF.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2++), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
  • 配列類似性
    Belongs to the Rho GDI family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARHGDIA antibody
    • fa96g11 antibody
    • GDIA 1 antibody
    • GDIA1 antibody
    • GDIR1_HUMAN antibody
    • MGC117248 antibody
    • NPHS8 antibody
    • Rho GDI 1 antibody
    • Rho GDI alpha antibody
    • Rho GDI antibody
    • Rho GDP dissociation inhibitor (GDI) alpha antibody
    • Rho GDP dissociation inhibitor 1 antibody
    • Rho GDP dissociation inhibitor alpha antibody
    • Rho GDP-dissociation inhibitor 1 antibody
    • Rho-GDI alpha antibody
    • RhoGDI 1 antibody
    • RhoGDI alpha antibody
    • RHOGDI antibody
    • RhoGDI1 antibody
    • wu:fa96g11 antibody
    • zgc:55554 antibody
    • zgc:77681 antibody
    see all

画像

  • All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution

    Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins)
    Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 105 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
    1: No phosphopeptide
    2: Phosphopeptide

参考文献

This product has been referenced in:
  • Oishi A  et al. Regulation of RhoA Signaling by the cAMP-dependent Phosphorylation of RhoGDIa. J Biol Chem 287:38705-15 (2012). WB . Read more (PubMed: 23012358) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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