Anti-RhoGDI (phospho S174) 抗体 (ab74142)
Key features and details
- Rabbit polyclonal to RhoGDI (phospho S174)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RhoGDI (phospho S174) antibody
RhoGDI 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RhoGDI (phospho S174) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human RhoGDI (phospho S174).
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ポジティブ・コントロール
- IHC-P: Human cervix carcinoma tissue. WB: HUVEC cells treated with EGF.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74142の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 23 kDa.
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IHC-P |
1/50 - 1/100.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 23 kDa. |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
配列類似性
Belongs to the Rho GDI family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 396 Human
- Entrez Gene: 192662 Mouse
- Entrez Gene: 360678 Rat
- Omim: 601925 Human
- SwissProt: P52565 Human
- SwissProt: Q99PT1 Mouse
- SwissProt: Q5XI73 Rat
- Unigene: 159161 Human
see all -
別名
- ARHGDIA antibody
- fa96g11 antibody
- GDIA 1 antibody
see all
画像
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All lanes : Anti-RhoGDI (phospho S174) antibody (ab74142) at 1/500 dilution
Lane 1 : HUVEC cells treated with EGF (200ng/ml, 30mins)
Lane 2 : HUVEC cells treated with EGF (200ng/ml, 30mins) with phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 105 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
Human cervix carcinoma tissue stained with ab74142 (1/50 dilution).
1: No phosphopeptide
2: Phosphopeptide
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab74142 は 2 報の論文で使用されています。
- Keller-Pinter A et al. The phosphomimetic mutation of syndecan-4 binds and inhibits Tiam1 modulating Rac1 activity in PDZ interaction-dependent manner. PLoS One 12:e0187094 (2017). PubMed: 29121646
- Oishi A et al. Regulation of RhoA Signaling by the cAMP-dependent Phosphorylation of RhoGDIa. J Biol Chem 287:38705-15 (2012). WB . PubMed: 23012358