製品の概要

  • 製品名Anti-RhoGAP antibody
    RhoGAP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RhoGAP
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the center region of Human RhoGAP (NP_001164.2).

  • ポジティブ・コントロール
    • 293 cell line lysate; Mouse cerebellum tissue lysates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab93375 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 171 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性The p190 RhoGAP protein is associated with p120 RasGAP in growth-factor stimulated and tyrosine kinase transformed cells. It functions as a GTPase - activating protein (GAP) for Rho and Rac family proteins, which are involved in regulating cytoskeletal actin and membrane ruffling. The antibody appears to couple signal transduction via Ras and Rho through its association with RasGAP. The presence of two adjacent SH2 domains in the p21 RAS GAP indicates that GAP might interact directly with tyrosine kinases. p190B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190B also has been shown to be tyrosine phosphorylated by cSrc and vSrc. Additionally the GAP domain of p190B is shown to attenuate signal transducing activity of Rac, Rho and CDC42.
    • 細胞内局在Cytoplasmic
    • 参照データベース
    • 別名
      • ARHGAP 5 antibody
      • ARHGAP5 antibody
      • p190 B antibody
      • p190 RhoGAP antibody
      • Rho GTPase activating protein 5 antibody
      • RhoGAP 5 antibody
      • RhoGAP antibody
      • RhoGAP5 antibody
      see all

    Anti-RhoGAP antibody 画像

    • All lanes : Anti-RhoGAP antibody (ab93375) at 1/100 dilution

      Lane 1 : 293 cell line lysate
      Lane 2 : Mouse cerebellum tissue lysate

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 171 kDa

    Anti-RhoGAP antibody (ab93375) 使用論文

    ab93375 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab93375.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"