製品の概要

  • 製品名Anti-RHOG antibody [2E6]
    RHOG 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2E6] to RHOG
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Hamster, Cow, Pig, Duck
  • 免疫原

    Recombinant fragment with tag (MW of the tag alone is 26 KDa): RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAV RYLECSALQQ DGVKEVFAEA VRAVLNPTPI KRGRSCILL, corresponding to amino acids 94-192 of Human RHOG. Note: the sequence provided does not include that of the tag.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名2E6
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: Use at an assay dependent dilution.
    sELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Predicted molecular weight: 21 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.
    • 配列類似性Belongs to the small GTPase superfamily. Rho family.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ARHG antibody
      • Ras homolog family member G antibody
      • Rho G antibody
      • Rho related GTP binding protein RhoG precursor antibody
      • Rho-related GTP-binding protein RhoG antibody
      • RHOG antibody
      • RHOG_HUMAN antibody
      see all

    Anti-RHOG antibody [2E6] 画像

    • Anti-RHOG antibody [2E6] (ab76508) at 1/500 dilution + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 21 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Detection limit for recombinant tagged RHOG is approximately 1ng/ml when used as a capture antibody at 1ug/well.

    Anti-RHOG antibody [2E6] (ab76508) 使用論文

    ab76508 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab76508.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"