製品の概要

  • 製品名Anti-RhoF antibody
    RhoF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RhoF
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Pig
  • 免疫原

    Recombinant fragment, corresponding to a region within internal sequence amino acids 16-156 of Human RhoF (AAH18208).

  • ポジティブ・コントロール
    • U87-MG whole cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab101349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.

ターゲット情報

  • 機能Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
  • 配列類似性Belongs to the small GTPase superfamily. Rho family.
  • 細胞内局在Cell membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARHF antibody
    • FLJ20247 antibody
    • Ras homolog gene family member F (in filopodia) antibody
    • Ras homolog gene family member F antibody
    • Rho F antibody
    • Rho family GTPase Rif antibody
    • Rho in filopodia antibody
    • Rho related GTP binding protein RhoF antibody
    • Rho-related GTP-binding protein RhoF antibody
    • Rhof antibody
    • RHOF_HUMAN antibody
    • RIF antibody
    see all

Anti-RhoF antibody 画像

  • IHC image of RhoF staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101349, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-RhoF antibody (ab101349) at 1/500 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size : 24 kDa
    12% SDS PAGE

Anti-RhoF antibody (ab101349) 使用論文

ab101349 has not yet been referenced specifically in any publications.

Product Wall

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team...

Read More

Thank you for your reply.

I am sorry that optimization hasn't helped improve the results. Would you like to receive a replacement vial as previous vials were working successfully?

Looking forward to your reply.

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

Vielen Dank für Ihren Anruf.

Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogen...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"