製品の概要

  • 製品名Anti-Rhodopsin antibody [RET-P1]
    Rhodopsin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [RET-P1] to Rhodopsin
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Membrane preparation from adult rat retina.

  • ポジティブ・コントロール
    • IMR-5 cells Brain or retina tissue sections.
  • 特記事項


    Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.40
    Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina.
  • ポリ/モノモノクローナル
  • クローン名RET-P1
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/2 - 1/4. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Staining protocol - boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 min.
WB Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Blot incubation - 2hrs at RT

IP Use at an assay dependent concentration. PubMed: 18974094

ターゲット情報

  • 機能Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • 組織特異性Rod shaped photoreceptor cells which mediates vision in dim light.
  • 関連疾患Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • 配列類似性Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • 翻訳後修飾Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • 細胞内局在Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Anti-Rhodopsin antibody [RET-P1] 画像

  • ab3267 at a 1/100 dilution staining Rhosopsin in murine retina by IHC-Wholemount. Tissue was fixed in 4% paraformaldehyde and permeabilized using Triton. The secondary used was an Atto488 conjugated goat polyclonal used at a 1/200 dilution.
    Rods stained with ab3267 (green) in whole mount retina of young adult mouse (p21), actin stained using phalloidin (red).

    See Abreview



  • Predicted band size : 39 kDa

    Image from Chucair-Elliott AJ et al., J Biol Chem 287:24092-102 (2012); Fig 1.; doi: 10.1074/jbc.M112.378240; July 13, 2012, The Journal of Biological Chemistry, 287, 24092-24102.

  • ab3267 staining Rhodopsin in Rat eyeball tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 20 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in 1% BSA in PBS) for 1 hours at 25°C. A HRP-conjugated Goat anti-mouse IgG polyclonal undiluted was used as the secondary antibody.
    Rods and cones of retinal outter granular cells were stained positively for rhodopsin. In normal conditions, synthesized rhodopsin is predominatly stored in the cellular projects, not in the cell bodies.

    See Abreview

  • Overlay histogram showing Y79 cells stained with ab3267 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3267, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Y79 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Rhodopsin antibody [RET-P1] (ab3267) 使用論文

This product has been referenced in:
  • Tzameret A  et al. Transplantation of human bone marrow mesenchymal stem cells as a thin subretinal layer ameliorates retinal degeneration in a rat model of retinal dystrophy. Exp Eye Res 118:135-44 (2014). IHC-P . Read more (PubMed: 24239509) »
  • Wavre-Shapton ST  et al. Conditional ablation of the choroideremia gene causes age-related changes in mouse retinal pigment epithelium. PLoS One 8:e57769 (2013). ICC/IF ; Mouse . Read more (PubMed: 23460904) »

See all 10 Publications for this product

Product Wall

The concentration of lot GR93754 for ab3267 is 200 ug/ml.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (eyeball)
Specification eyeball
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2012

Thank you for your enquiry.

I can confirm that recent trials did not show any influence on product performance after an additional freeze thaw cycle and so we advise customers that they can store this antibody frozen at -20oC now.

Read More

Application IHC - Wholemount
Sample Mouse Tissue (retina)
Specification retina
Username

Dr. Vincent Michel

Verified customer

投稿 Mar 26 2012

Application Immunohistochemistry free floating
Sample House Sparrow Cell (brain)
Specification brain
Username

Dr. Gang Wang

Verified customer

投稿 Mar 01 2011

Thank you for your enquiry. It may indeed be possible to use antibodies in IHC-Fr (frozen sections) when they are already working in IHC-P. This is likely as the fact they work in IHC-P means they have the capacity to recognise the antigen in its na...

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Thank you for your enquiry. The antibody ab3267 has been tested in immunocytochemistry in IF in IMR-5 cells, unfortunately we do not have information about the dilution used but since the antibody worked at 4ug /ml for 30 min at RT in paraffin sections...

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This data is unknown.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"