製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Rhodopsin
  • 特異性
    Detects Rhodopsin from bovine retina samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab3424 appears to recognize Red Opsin rather than Rhodopsin.
  • アプリケーション
    適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Cow
    交差が予測される動物種: Rat, Rabbit, Cat, Dog, Human, Cynomolgus monkey, Chinese hamster, African bush elephant非交差種: Sheep
  • 免疫原

    Synthetic peptide corresponding to Cow Rhodopsin aa 338-348.
    Sequence:

    SKTETSQVAPA


    (Peptide available as ab4967)

  • ポジティブ・コントロール
    • Bovine outer rod segment extracts.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein rhodopsin absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble proteins arrestin.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).Can be blocked with Rhodopsin peptide (ab4967).

By Western blot, this antibody detects an ~36 kDa protein representing rhodopsin from bovine outer rod segment extracts.

IHC-Fr Use at an assay dependent concentration. PubMed: 22743318

ターゲット情報

  • 機能
    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • 組織特異性
    Rod shaped photoreceptor cells which mediates vision in dim light.
  • 関連疾患
    Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • 翻訳後修飾
    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • 細胞内局在
    Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

Anti-Rhodopsin antibody 画像

  • Immunohistochemistry (Frozen sections) image of anti-Rhodopsin antibody (ab3424) staining on a Zebrafish eye. This tissue section was stained with anti-Rhodopsin antibody ab3424 (green) and anti-Rhodopsin monoclonal antibody ab5417 (red) for 16 hours at 4°C. Nuclei were stained with DAPI (blue). This image suggests that ab3424 (like ab5417) may detect Red Opsin and not Rhodopsin in the Zebrafish. For more information please see Yin J. et al., 2012.

    See Abreview

Anti-Rhodopsin antibody (ab3424) 使用論文

This product has been referenced in:
  • MacDonald RB  et al. Müller glia provide essential tensile strength to the developing retina. J Cell Biol 210:1075-83 (2015). Read more (PubMed: 26416961) »
  • Yin J  et al. The 1D4 antibody labels outer segments of long double cone but not rod photoreceptors in zebrafish. Invest Ophthalmol Vis Sci 53:4943-51 (2012). IHC-Fr ; Zebrafish . Read more (PubMed: 22743318) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.4% triton
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Brian Maunze

Verified customer

投稿 Jul 20 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Zebrafish Tissue sections (Long double cone photoreceptor cells in zebrafish)
Specification
Long double cone photoreceptor cells in zebrafish
Permeabilization
Yes - PBST
Fixative
Paraformaldehyde
Username

Dr. Jun Yin

Verified customer

投稿 Jul 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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