製品の概要

  • 製品名
    Anti-Rhodopsin antibody [1D4]
    Rhodopsin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1D4] to Rhodopsin
  • 由来種
    Mouse
  • 特異性
    ab5417 detects Rhodopsin from Human and Bovine retinal samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab5417 appears to recognize Red Opsin rather than Rhodopsin.
  • アプリケーション
    適用あり: IHC-FoFr, IHC-Fr, ELISA, ICC, IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Zebrafish, Amphibian
    交差が予測される動物種: Rabbit
  • 免疫原

    Other Immunogen Type corresponding to Cow Rhodopsin.

  • エピトープ
    The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 一次抗体 備考
    Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.
  • ポリ/モノ
    モノクローナル
  • クローン名
    1D4
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/1000. PubMed: 19587120
IHC-Fr Use at an assay dependent concentration. PubMed: 22743318
ELISA Use at an assay dependent concentration.
ICC 1/1000.
IP Use at an assay dependent concentration.

Use at an assay dependent dilution.

WB 1/1000. Detects a band of approximately 40 kDa.
IHC-P 1/100 - 1/1000.

ターゲット情報

画像

  • Immunohistochemical analysis of formalin-fixed mouse retinal tissue, labelling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment. A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature. The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

  • Immunohistochemical analysis of paraffin-embedded mouse retinal tissue labelling Rhodopsin with ab5417. Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrival using 10mM sodium citrate at pH 6.0, then the sample was microwaved for 8 to 15 minutes. Subsequent to retrival the retinal tissue was blocked for 15 minutes at room temperature with 3% hydrogen peroxide. The sample was then incubated with ab5417 in 3% BSA-PBS at 4°C at a dilution of 1:1000, overnight. Hematoxylin was used to counterstain the tissue. The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.

  • Immunohistochemistry (Frozen sections) image of anti-Rhodopsin antibody (ab5417) staining on a Zebrafish eye. This tissue section was stained with anti-Rhodopsin antibody (green) and Red Opsin antibody (red) for 16 hours at 4°C. Nuclei were stained with DAPI (blue). This image suggests that ab5417 detects Red Opsin and not Rhodopsin in the Zebrafish. For more information please see Yin J. et al., 2012.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed human retinal tissue, labelling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment. A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature. The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

  • Anti-Rhodopsin antibody [1D4] (ab5417) at 1/500 dilution + HL60 cell lysate at 25 µg

    Observed band size: 40 kDa (why is the actual band size different from the predicted?)

参考文献

This product has been referenced in:
  • Fliesler SJ  et al. Prevention of Retinal Degeneration in a Rat Model of Smith-Lemli-Opitz Syndrome. Sci Rep 8:1286 (2018). Rat . Read more (PubMed: 29352199) »
  • Ballister ER  et al. A live cell assay of GPCR coupling allows identification of optogenetic tools for controlling Go and Gi signaling. BMC Biol 16:10 (2018). Read more (PubMed: 29338718) »

See all 30 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (neuroblast)
Specification
neuroblast
Blocking step
BSA as blocking agent for 50 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Zebrafish Tissue sections (Long double cone (red cone) photoreceptor cells)
Specification
Long double cone (red cone) photoreceptor cells
Permeabilization
Yes - PBST
Fixative
Paraformaldehyde
Username

Dr. Jun Yin

Verified customer

投稿 Jul 02 2013

Thank you very much for your call today. It was a pleasure speaking to you. I've created the discount code below, and if you have any further questions about redeeming the code or submitting the Abreview, please let me know and I'll be happy to help. <...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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