製品の概要

  • 製品名Anti-Rho antibody [EP487Y]
    Rho 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP487Y] to Rho
  • 特異性This antibody is specific for Rho. It is also expected to detect RhoA, RhoB and RhoC.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Rho (N terminal).

  • ポジティブ・コントロール
    • HL60 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名EP487Y
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab40673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/250.
WB 1/1000 - 1/5000. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ターゲット情報

  • 機能Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  • 配列類似性Belongs to the small GTPase superfamily. Rho family.
  • ドメインThe basic-rich region is essential for yopT recognition and cleavage.
  • 翻訳後修飾Substrate for botulinum ADP-ribosyltransferase.
    Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
    AMPylation at Tyr-34 and Thr-37 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
    Phosphorylation by PRKG1 at Ser-188 inactivates RHOA signaling.
    Ubiquitinated by the BCR(BACURD1) and BCR(BACURD2) E3 ubiquitin ligase complexes, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration.
  • 細胞内局在Cell membrane. Cytoplasm > cytoskeleton. Cleavage furrow. Cytoplasm > cell cortex. Midbody. Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in a activated form and in a myosin- and actin-independent manner.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARH12 antibody
    • ARHA antibody
    • H12 antibody
    • ras homolog gene family member A antibody
    • ras homolog gene family member B antibody
    • ras homolog gene family member C antibody
    • Rho cDNA clone 12 antibody
    • RHO12 antibody
    • RHOA antibody
    • RHOA_HUMAN antibody
    • rhob antibody
    • rhoc antibody
    • RHOH12 antibody
    • Small GTP binding protein RhoA antibody
    • Transforming protein RhoA antibody
    see all

Anti-Rho antibody [EP487Y] 画像

  • All lanes : Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified)

    Lane 1 : A431 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 21 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified) + Mouse kidney tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 21 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified) + C6 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 21 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-Rho antibody [EP487Y] (ab40673) at 1/5000 dilution (purified) + HL-60 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 21 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified) + MDA-MB-435 at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L)

    Predicted band size : 21 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human thyroid carcinoma with purified ab40673 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunofluorescence staining of MCF7 cells with purified ab40673 at a working dilution of 1 in 250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab40673 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.

  • Anti-Rho antibody [EP487Y] (ab40673) (unpurified) + 10ug HL60 cell lysate

    Predicted band size : 21 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • Unpurified ab40673 at a 1:100 dilution staining Rho in human prostate carcinoma tissue.

    ab40673 at a 1:100 dilution staining Rho in human prostate carcinoma tissue.

Anti-Rho antibody [EP487Y] (ab40673) 使用論文

This product has been referenced in:
  • Nguyen H  et al. Interleukin-17 causes Rho-kinase-mediated endothelial dysfunction and hypertension. Cardiovasc Res 97:696-704 (2013). WB ; Rat . Read more (PubMed: 23263331) »
  • Huesa G  et al. Altered distribution of RhoA in Alzheimer's disease and AbetaPP overexpressing mice. J Alzheimers Dis 19:37-56 (2010). Read more (PubMed: 20061625) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Nov 10 2014



La concentration du RabMab anti-Rho ab40673 lot GR141864-2 est 0.692 mg/ml.

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Caenorhabditis elegans Cell lysate - whole cell (Embryonic extract (40ug) in lanes 1-3, 80 worm mic)
Loading amount 40 µg
Specification Embryonic extract (40ug) in lanes 1-3, 80 worm mic
Gel Running Conditions Non-reduced Denaturing (12% PA)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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投稿 Sep 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (J774 cell line)
Loading amount 10 µg
Specification J774 cell line
Treatment co-culture with C. Albicans
Gel Running Conditions Reduced Denaturing (14% gel)
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Feb 25 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"