製品の概要

  • 製品名Anti-RHEB antibody [Rheb 5D5]
    RHEB 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Rheb 5D5] to RHEB
  • アプリケーション適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human, Pig
  • 免疫原

    Human Rheb GST fusion.

  • ポジティブ・コントロール
    • HeLa or 293T cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 24790108
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
  • 組織特異性Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
  • 配列類似性Belongs to the small GTPase superfamily. Rheb family.
  • 翻訳後修飾Farnesylation is important for efficiently activating mTORC1-mediated signaling.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ras homolog enriched in brain 2, formerly antibody
    • GTP binding protein Rheb antibody
    • GTP-binding protein Rheb antibody
    • MGC111559 antibody
    • Ras homolog enriched in brain 2 antibody
    • Ras homolog enriched in brain antibody
    • RHEB 2 antibody
    • Rheb antibody
    • RHEB_HUMAN antibody
    • RHEB2 antibody
    • RHEB2, formerly antibody
    see all

Anti-RHEB antibody [Rheb 5D5] 画像

  • LLC-PK1 cells were grown on Transwell cell culture inserts, serum-starved overnight, and then kept in the absence (a) or presence (b) of 20.0 mg/ml albumin for 20 minutes. Blue (DAPI) - nuclei, Green - RAPTOR, Red - RHEB labelled with ab97896. Scale bar = 5 μm.

Anti-RHEB antibody [Rheb 5D5] (ab97896) 使用論文

This product has been referenced in:
  • Peruchetti DB  et al. Mis-regulation of Mammalian Target of Rapamycin (mTOR) Complexes Induced by Albuminuria in Proximal Tubules. J Biol Chem 289:16790-801 (2014). ICC/IF ; Pig . Read more (PubMed: 24790108) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab97896.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"