製品の概要

  • 製品名
    Anti-RHEB antibody [Rheb 5D5]
    RHEB 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Rheb 5D5] to RHEB
  • アプリケーション
    適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human, Pig
  • 免疫原

    Human Rheb GST fusion.

  • ポジティブ・コントロール
    • HeLa or 293T cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 24790108
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
  • 組織特異性
    Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
  • 配列類似性
    Belongs to the small GTPase superfamily. Rheb family.
  • 翻訳後修飾
    Farnesylation is important for efficiently activating mTORC1-mediated signaling.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ras homolog enriched in brain 2, formerly antibody
    • GTP binding protein Rheb antibody
    • GTP-binding protein Rheb antibody
    • MGC111559 antibody
    • Ras homolog enriched in brain 2 antibody
    • Ras homolog enriched in brain antibody
    • RHEB 2 antibody
    • Rheb antibody
    • RHEB_HUMAN antibody
    • RHEB2 antibody
    • RHEB2, formerly antibody
    see all

画像

  • LLC-PK1 cells were grown on Transwell cell culture inserts, serum-starved overnight, and then kept in the absence (a) or presence (b) of 20.0 mg/ml albumin for 20 minutes. Blue (DAPI) - nuclei, Green - RAPTOR, Red - RHEB labelled with ab97896. Scale bar = 5 μm.

参考文献

This product has been referenced in:
  • Peruchetti DB  et al. Mis-regulation of Mammalian Target of Rapamycin (mTOR) Complexes Induced by Albuminuria in Proximal Tubules. J Biol Chem 289:16790-801 (2014). ICC/IF ; Pig . Read more (PubMed: 24790108) »

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab97896.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録