The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Can be blocked with RHEB peptide (ab39862).
Post-translationally modified Rheb is sometimes observed at higher molecular weight.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
組織特異性Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
配列類似性Belongs to the small GTPase superfamily. Rheb family.
翻訳後修飾Farnesylation is important for efficiently activating mTORC1-mediated signaling.