The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Use at an assay dependent concentration. PubMed: 24648344
関連性RHBDF1 is a seven-transmembrane protein with a long N-terminal cytoplasmic extension that comprises half of the polypeptide sequence, and is found in the endoplasmic reticulum and Golgi, but not on the cell surface. RHBDF1 has a pivotal role in sustaining growth signals in epithelial cancer cells and thus may serve as a therapeutic target for treating epithelial cancers.
epidermal growth factor receptor related sequence antibody
FLJ 2235 antibody
FLJ 22357 antibody
gene 89 antibody
gene 90 antibody
h Dist 1 antibody
hDist 1 antibody
RHBDF 1 antibody
rhomboid 5 homolog 1 (Drosophila) antibody
Rhomboid 5 homolog 1 antibody
rhomboid family 1 antibody
Anti-RHBDF1 antibody 画像
Western blot - RHBDF1 antibody (ab81342)
Anti-RHBDF1 antibody (ab81342) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 97 kDa
Anti-RHBDF1 antibody (ab81342) 使用論文
This product has been referenced in:
Zhou Z et al. Human rhomboid family-1 suppresses oxygen-independent degradation of hypoxia-inducible factor-1a in breast cancer. Cancer Res74:2719-30 (2014).
Read more (PubMed: 24648344) »