製品の概要

  • 製品名Anti-RGS4 antibody
    RGS4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RGS4
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Peptide: N-

    LRSAKDMKHRLGFL

    -C (aa 13-26) fused to rabbit serum albumin.

  • ポジティブ・コントロール
    • WB: human frontal cortex lysate (see Erdely et al)

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500. Detects a band of approximately 28, 40 kDa (predicted molecular weight: 28 kDa). (see Erdely et al and recommended protocol). Block membrane in 5% milk and incubate antibody overnight in TBST containing 1% milk. Erdely HA et al noted that "...a much lighter intensity band was also detected at approximately 40 kDa, which unlike the band at 28 kDa, was not blocked by the blocking peptide. Therefore, the 40 kDa band was considered to be nonspecific."

ターゲット情報

  • 機能Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein.
  • 関連疾患Genetic variation in RGS4 is associated with susceptibility to schizophrenia type 9 (SCZD9) [MIM:604906]. A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.
  • 配列類似性Contains 1 RGS domain.
  • 翻訳後修飾Palmitoylated on Cys-2 and/or Cys-12.
    Phosphorylated by cyclic GMP-dependent protein kinase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC2124 antibody
    • MGC60244 antibody
    • Regulator of G protein signalling 4 antibody
    • Regulator of G-protein signaling 4 antibody
    • RGP 4 antibody
    • RGP4 antibody
    • RGS 4 antibody
    • RGS4 antibody
    • RGS4_HUMAN antibody
    • Schizophrenia disorder 9 antibody
    • SCZD 9 antibody
    • SCZD9 antibody
    see all

Anti-RGS4 antibody 画像

  • IHC image of ab9964 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9964, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-RGS4 antibody (ab9964) 使用論文

This product has been referenced in:
  • Li SJ  et al. Splicing factor transformer-2ß (Tra2ß) regulates the expression of regulator of G protein signaling 4 (RGS4) gene and is induced by morphine. PLoS One 8:e72220 (2013). WB ; Rat . Read more (PubMed: 23977258) »
  • Chen Y  et al. Neurabin scaffolding of adenosine receptor and RGS4 regulates anti-seizure effect of endogenous adenosine. J Neurosci 32:2683-95 (2012). Mouse . Read more (PubMed: 22357852) »

See all 6 Publications for this product

Product Wall

Thank you very much for your reply.

The vial of ab9962 will be transferring from our stock in the UK, so this free of chargereplacementis expected to arrive around October 2 on the order ***.

Please keep me updated about the results...

Read More

Thank you very much for your reply.

I will send this out promptly. Could you tell me if you'd like a biotin or HRP conjugated secondary antibody? We have ab5744 (biotin) or ab5745 (HRP)-

http://www.abcam.com/Armenian-Hamster-IgG-sec...

Read More

Thank you very much for your reply.

I would suggest trying a different antibody at this point since two recent vials of ab9964 have not worked and it may take some time to resolve the issue with this antibody. We do have two other RGS4 antibo...

Read More

Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge replacement vial from a new lot on the order ***, which will arrive shortly. Please keep me updated abou...

Read More

Thank you for your enquiry. The difference in dilutions may be due to the levels of protein in the samples, therefore it is possible that Erdely et al use samples which contain high levels of RGS4, which means the antibody will give very strong bands...

Read More

Thank you for your enquiry. Here is the most detailed protocol that I have for blocking experiments. If you follow this protocol and you are not blocking any bands something is defintely wrong. Please let me know how you proceed and your results. ...

Read More

Thank you for your enquiry. Thank you also for taking the time to complete our western blot questionaire. I have examined your protocol and it seems not disimilar to abcam's recommended protocol. Your blots are indeed peculiar. Please can you tell m...

Read More

Thank you for your enquiry. The concentration for this antibody is undeterminable, as it is provided as whole antiserum. Please contact us again if you require further information.

Thank you for your enquiry regarding a blocking peptide for ab9964. Unfortunately, we don't have a blocking peptide available within our catalogue for this anitibody. I'm sorry for any inconvenience that this may cause to you. In the future if you cann...

Read More

Thank you for your enquiry. RGS4 migrates just below the 30 kDa marker on SDS gels. At high concentration multiple bands are often seen, we would suggest decreasing the primary and/or the secondary antibody concentration and/or the incubation perio...

Read More

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"