製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RGS3
  • アプリケーション
    適用あり: IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    E. coli expressed human RGS 3 isoform 1 (amino acids 401-519).

  • 特記事項


    RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 132 kDa).

ターゲット情報

  • 機能
    Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases.
  • 配列類似性
    Contains 1 C2 domain.
    Contains 1 PDZ (DHR) domain.
    Contains 1 RGS domain.
  • 翻訳後修飾
    Phosphorylated by cyclic GMP-dependent protein kinase.
    ISGylated.
  • 細胞内局在
    Cytoplasm. Nucleus. Cell membrane. Long isoforms are cytoplasmic and associated with the plasma membrane. Short isoforms are nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C2PA antibody
    • FLJ20370 antibody
    • FLJ31516 antibody
    • FLJ90496 antibody
    • PDZ RGS 3 antibody
    • PDZ RGS3 antibody
    • Regulator of G protein signaling 3 antibody
    • Regulator of G protein signalling 3 antibody
    • Regulator of G-protein signaling 3 antibody
    • RGP 3 antibody
    • RGP3 antibody
    • RGS 3 antibody
    • RGS3 antibody
    • RGS3_HUMAN antibody
    see all

画像

  • Lane 1 : Anti-RGS3 antibody (ab2564)
    Lane 2 : Anti-RGS3 antibody (ab2564) at 1 µg/ml

    Lane 1 : mock
    Lane 2 : transfected HEK293 cells (right lane)


    Predicted band size : 132 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

参考文献

ab2564 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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