製品の概要

  • 製品名Anti-RGS20 antibody
    RGS20 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to RGS20
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment: NQEDQRPTIA SHELRADLPT WEESPAPTLE EVNAWAQSFD KLMVTPAGRN AFREFLRTEF SEENMLFWMA CEELKKEANK NIIEEKARII YEDYISILS, corresponding to amino acids 78-177 of Human RGS20 with tag (NP_003693).

  • ポジティブ・コントロール
    • NIH3T3 cell lysate; Recombinant fragment of human RGS20.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 44 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.
  • 組織特異性Isoform 5 is expressed in brain at high levels in the caudate nucleus and temporal lobe.
  • 配列類似性Contains 1 RGS domain.
  • 翻訳後修飾Fatty acylated. Heavily palmitoylated in the cysteine string motif.
    N- and O-glycosylated in synapsomal membranes.
    Serine phosphorylated in synapsomal membranes.
    Sumoylated by SUMO1 and SUM02 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits.
  • 細胞内局在Membrane. Nucleus. Cytoplasm. Shuttles between the cytoplasm/cell membrane and the nucleus (By similarity). Anchored to the membrane through palmitoylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • G(z)GAP antibody
    • Gz selective GTPase activating protein antibody
    • Gz-GAP antibody
    • Gz-selective GTPase-activating protein antibody
    • HGNC:14600 antibody
    • Regulator of G protein signaling 20 antibody
    • Regulator of G-protein signaling 20 antibody
    • Regulator of G-protein signaling Z1 antibody
    • Regulator of Gz selective protein signaling 1 antibody
    • Regulator of Gz-selective protein signaling 1 antibody
    • RGS20 antibody
    • RGS20_HUMAN antibody
    • RGSZ1 antibody
    • ZGAP1 antibody
    see all

Anti-RGS20 antibody 画像

  • Anti-RGS20 antibody (ab77009) at 5 µg/ml + Recombinant fragment of human RGS20 at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-RGS20 antibody (ab77009) at 5 µg/ml + NIH3T3 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 44 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-RGS20 antibody (ab77009) 使用論文

ab77009 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"