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Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RGS2.
(Peptide available as ab36560.)
Our Abpromise guarantee covers the use of ab36561 in the following tested applications.
ab36561 staining RGS2 in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.