製品の概要

  • 製品名Anti-RGS16 (phospho Y168) antibody
    RGS16 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RGS16 (phospho Y168)
  • 特異性ab74155 detects endogenous levels of RGS16 only when phosphorylated at tyrosine 168 (Human: Tyr168; Mouse: Tyr167).
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human RGS16 around the phosphorylation site of tyrosine 168 (D-S-YP-P-R).

  • ポジティブ・コントロール
    • Extracts from COS7 cells treated with heat shock.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab74155 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
ELISA 1/10000.

ターゲット情報

  • 機能Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. May play a role in regulating the kinetics of signaling in the phototransduction cascade.
  • 組織特異性Abundantly expressed in retina with lower levels of expression in most other tissues.
  • 配列類似性Contains 1 RGS domain.
  • 翻訳後修飾Palmitoylated on Cys-2 and/or Cys-12.
    Phosphorylation on Tyr-168 upon EGFR stimulation. Enhanced GTPase accelerating (GAP) activity on G(i)-alpha.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A28 RGS14 antibody
    • A28 RGS14P antibody
    • A28-RGS14P antibody
    • HGNC:9997 antibody
    • hRGS-r antibody
    • OTTHUMP00000033147 antibody
    • Regulator of G protein signaling 16 antibody
    • Regulator of G protein signalling 16 antibody
    • Regulator of G-protein signaling 16 antibody
    • Retinal-specific RGS antibody
    • Retinally abundant regulator of G protein signaling antibody
    • Retinally abundant regulator of G-protein signaling antibody
    • RGS 16 antibody
    • RGS R antibody
    • RGS-R antibody
    • Rgs14 antibody
    • RGS16 antibody
    • RGS16_HUMAN antibody
    • RGSR antibody
    see all

Anti-RGS16 (phospho Y168) antibody 画像

  • All lanes : Anti-RGS16 (phospho Y168) antibody (ab74155) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells treated with heat shock
    Lane 2 : Extracts from COS7 cells treated with heat shock with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

Anti-RGS16 (phospho Y168) antibody (ab74155) 使用論文

ab74155 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab74155.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"