製品の概要

  • 製品名Anti-RGNEF antibody
    RGNEF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RGNEF
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen: GTAHTEAQQS FMSPSSSCAS NLNLSFGWHG FEKEQSHLKK RSSSLDALDA DSEGEGHSEP SHICYTPGSQ SSSRTGIPSG DELDSFETNT EPDFNI, corresponding to amino acids 435-530 of Human RGNEF according to Q8N1W1

  • ポジティブ・コントロール
    • Human duodenum tissue, Human U-2 OS cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

ターゲット情報

  • 機能Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.
  • 配列類似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • 翻訳後修飾Phosphorylated on tyrosine upon stimulation of cells by laminin.
  • 細胞内局在Cytoplasm. Cell membrane. Colocalizes with the microtubule radial and cortical systems.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 190 kDa guanine nucleotide exchange factor antibody
    • guanine nucleotide exchange factor, 190-KD antibody
    • KIAA1998 antibody
    • p190-RhoGEF antibody
    • p190RhoGEF antibody
    • RGNEF antibody
    • RGNEF, mouse, homolog of antibody
    • RGNEF_HUMAN antibody
    • Rho guanine nucleotide exchange factor (GEF) 28 antibody
    • rho guanine nucleotide exchange factor 28 antibody
    • Rho interacting protein 2 antibody
    • Rho-guanine nucleotide exchange factor antibody
    • RhoGEF antibody
    • RIP2 antibody
    see all

Anti-RGNEF antibody 画像

  • ab122399, at a 1/200 dilution, staining RGNEF in glandular cells of paraffin-embedded Human duodenum tissue by immunohistochemistry.
  • ab122399, at 4 µg/ml, staining RGNEF (in green) in PFA/Triton X-100 treated Human cell line U-2 OS by immunofluorescence.

Anti-RGNEF antibody (ab122399) 使用論文

ab122399 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry
Sample Mouse Cultured Cells (3T3 Fibroblasts)
Permeabilization Yes - 0.1% Triton-X
Specification 3T3 Fibroblasts
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Paraformaldehyde
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投稿 Jun 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"