製品の概要

  • 製品名Anti-RFX5 antibody
    RFX5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RFX5
  • 特異性Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 85 kDa and 55 kDa.
  • アプリケーション適用あり: WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH.

  • 特記事項Western blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)This product was prepared from monospecific antiserum by delipidation, immunoadsorption against an E.coli lysate immobilized on agarose beads and immunoaffinity chromatography using a Protein G resin followed by extensive dialysis against buffer.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab7543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
EMSA 1/0.5 - 1/1.

ターゲット情報

  • 機能Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
  • 組織特異性Ubiquitous.
  • 関連疾患Defects in RFX5 are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections.
  • 配列類似性Belongs to the RFX family.
    Contains 1 RFX-type winged-helix DNA-binding domain.
  • ドメインThe N-terminus is required for dimer formation, association with RFXANK and RFXAP, assembly of the RFX complex, and for binding of this complex to its X box target site in the MHC-II promoter. The C-terminus mediates cooperative binding between the RFX complex and NF-Y.
  • 翻訳後修飾Phosphorylated.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA binding protein RFX 5 antibody
    • DNA binding protein RFX5 antibody
    • DNA-binding protein RFX5 antibody
    • Influences HLA class II expression antibody
    • Regulatory factor X 5 antibody
    • Regulatory factor X subunit 5 antibody
    • Regulatory factor X, 5 (influences HLA class II expression) antibody
    • RFX 5 antibody
    • RFX5 antibody
    • Rfx5 protein antibody
    • RFX5_HUMAN antibody
    see all

Anti-RFX5 antibody (ab7543) 使用論文

ab7543 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"