製品の概要

  • 製品名Anti-RFWD3 antibody
    RFWD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RFWD3
  • アプリケーション適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Immunogen corresponds to a region within amino acids 75-125 of Human RFWD3 (NP_060594.3).

  • ポジティブ・コントロール
    • Hela whole cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Contains 1 RING-type zinc finger.
      Contains 3 WD repeats.
    • 翻訳後修飾Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BC027246 antibody
      • E3 ubiquitin protein ligase RFWD3 antibody
      • E3 ubiquitin-protein ligase RFWD3 antibody
      • FLJ10520 antibody
      • MGC27888 antibody
      • RFWD3 antibody
      • RFWD3_HUMAN antibody
      • Ring finger and WD repeat domain 3 antibody
      • RING finger and WD repeat domain containing protein 3 antibody
      • RING finger and WD repeat domain-containing protein 3 antibody
      • RING finger protein 201 antibody
      • RNF201 antibody
      see all

    Anti-RFWD3 antibody 画像

    • An anti RFWD3 antibody, at 1 µg/ml, staining RFWD3 in HeLa whole cell lysate immunoprecipitated using ab99306 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

    Anti-RFWD3 antibody (ab99306) 使用論文

    ab99306 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab99306.
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