Anti-RFWD3 抗体 (ab99306)
Key features and details
- Rabbit polyclonal to RFWD3
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RFWD3 antibody
RFWD3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RFWD3 -
由来種
Rabbit -
アプリケーション
適用あり: IPmore details -
種交差性
交差種: Human -
免疫原
Immunogen corresponds to a region within amino acids 75-125 of Human RFWD3 (NP_060594.3).
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ポジティブ・コントロール
- Hela whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99306の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 RING-type zinc finger.
Contains 3 WD repeats. -
翻訳後修飾
Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 55159 Human
- Omim: 614151 Human
- SwissProt: Q6PCD5 Human
- Unigene: 567525 Human
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別名
- BC027246 antibody
- E3 ubiquitin protein ligase RFWD3 antibody
- E3 ubiquitin-protein ligase RFWD3 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab99306 は 1 報の論文で使用されています。
- Lai Y et al. HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation. Sci Rep 9:14257 (2019). PubMed: 31582797