製品の概要

  • 製品名Anti-RFP2 antibody
    RFP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RFP2
  • 特異性This antibody is specific for RFP2.
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    A region within synthetic peptide: DTLETSKRKS LQLLTKDSDK VKEFFEKLQH TLDQKKNEIL SDFETMKLAV, corresponding to amino acids 162-211 of Human RFP2

  • ポジティブ・コントロール
    • Fetal lung lysate, human kidney tissue and human lung tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab42502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/62500.
WB Use a concentration of 0.125 µg/ml. Detects a band of approximately 39, 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能E3 ubiquitin ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 RING-type zinc finger.
  • ドメインThe coiled-coil domain is required for the induction of autophagy during endoplasmic reticulum (ER) stress.
    The RING-type zinc finger is required for auto-polyubiquitination.
    The C-terminal domain transmembrane domain is indispensable for the localization to the ER.
  • 翻訳後修飾Auto-ubiquitinated; requires the RING-type zinc finger. Auto-polyubiquitination leads to proteasomal degradation.
  • 細胞内局在Endoplasmic reticulum membrane. Concentrates and colocalizes with p62/SQSTM1 and ZFYVE1 at the perinuclear endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B cell chronic lymphocytic leukemia tumor suppressor Leu5 antibody
    • B-cell chronic lymphocytic leukemia tumor suppressor Leu5 antibody
    • CAR antibody
    • DLEU5 antibody
    • E3 ubiquitin-protein ligase TRIM13 antibody
    • HGNC:9976 antibody
    • LEU5 antibody
    • Leukemia associated protein 5 antibody
    • Leukemia-associated protein 5 antibody
    • Putative tumor suppressor RFP2 antibody
    • Ret finger protein 2 antibody
    • RING finger protein 77 antibody
    • RNF77 antibody
    • TRI13_HUMAN antibody
    • Trim13 antibody
    • Tripartite motif protein 13 antibody
    • Tripartite motif-containing protein 13 antibody
    see all

Anti-RFP2 antibody 画像

  • Anti-RFP2 antibody (ab42502) at 0.125 µg/ml + Fetal lung lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 39& 47 kDa (why is the actual band size different from the predicted?)
  • ab42502 at 4.0µg/ml staining RFP2 in human alveolar cells, lung tissue.
  • ab42502 at 4.0µg/ml staining RFP2 in human epithelial cells of collecting tubule in kidney tissue.

Anti-RFP2 antibody (ab42502) 使用論文

ab42502 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"