Anti-RFP 抗体 [RF5R] (ab125244)

製品の概要

  • 製品名
    Anti-RFP antibody [RF5R]
    RFP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [RF5R] to RFP
  • 特異性
    Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DsRed, mCherry, mOrange, etc.
  • アプリケーション
    適用あり: WB, ICC/IF, ELISA, Dot blot, IPmore details
  • 種交差性
    交差種: Sea anenome
  • 免疫原

    Synthetic peptide corresponding to Sea anenome RFP (N terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    VNGHEFEIEGEGEGR

  • ポジティブ・コントロール
    • Transfected HEK293 cell lysate.
  • 特記事項
    Store at 4°C (add 0.1% NaN3) for several days to weeks.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    ab125244 is purified by protein A affinity chromatography from Mouse ascites fluid.
  • ポリ/モノ
    モノクローナル
  • クローン名
    RF5R
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab125244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/3000. Predicted molecular weight: 26 kDa. Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins.
ICC/IF 1/500 - 1/2000.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
  • 参照データベース
    • 別名
      • DsRed antibody
      • GFP like chromoprotein antibody
      • red fluorescent protein antibody
      • RFP antibody

    Anti-RFP antibody [RF5R] 画像

    • All lanes : Anti-RFP antibody [RF5R] (ab125244) at 1/1000 dilution

      Lane 1 : HEK293 cell lysate, non-transfected
      Lane 2 : HEK293 cell lysate, Turbo-RFP-transfected
      Lane 3 : HEK293 cell lysate, DsRed-transfected

      Developed using the ECL technique

      Predicted band size : 26 kDa

    Anti-RFP antibody [RF5R] (ab125244) 使用論文

    ab125244 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Tribolium castaneum Embryo (Wholemount)
    Specification
    Wholemount
    Username

    Abcam user community

    Verified customer

    投稿 Aug 16 2013

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