製品の概要

  • 製品名Anti-RFP antibody
    RFP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RFP
  • 特異性ab62341 recognizes RFP and has been shown to react with tdTomato.
  • アプリケーション適用あり: IHC-P, IHC-FrFl, IHC-Fr, WB, IP, ICC/IFmore details
  • 免疫原

    Recombinant full length protein corresponding to RFP.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 23300543
IHC-FrFl Use at an assay dependent concentration. PubMed: 27581371
IHC-Fr Use at an assay dependent concentration. PubMed: 20889618
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP Use a concentration of 10 - 20 µg/ml. PubMed: 19704022
ICC/IF 1/100. PubMed: 19474334

ターゲット情報

  • 関連性Red fluorescent protein cloned from Entacmaea quadricolor eqFP611 (Uniprot IDQ8ISF8)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
  • 別名
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • Red fluorescent protein eqFP611 antibody
    • RFP antibody

Anti-RFP antibody 画像

  • ab62341, at 1/100, staining chicken midbrain cells by Immunocytochemistry/ Immunofluorescence.The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody for 3 hours. An Alexa fluor® 568 goat polyclonal to rabbit Ig, diluted 1/300, was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-RFP antibody (ab62341) at 1/2000 dilution

    Lane 1 : Whole cell lysate prepared from pDsRed-Monomer transfected HEK293 cells
    Lane 2 : Whole cell lysate prepared from pDsRed-Monomer transfected HEK293 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated anti-rabbit at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

    Image courtesy of an anonymous Abreview.

  • Immunohistochemical analysis of paraffin-embedded HT29 cells tissue labeling RFP with ab62341 at 1400 dilution,followed by Goat Anti-Rabbit (HRP) (ab62341)

  • ab62341 staining RFP in rat oligodendrocytes transfected with recombinant human adenovirus type 5 expressing Red Fluorescent Protein (RFP) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with formaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 5% serum for 10 minutes at 25°C. Samples were then incubated with ab62341 at a 1/300 dilution for 1 hour at 25°C. The secondary used was an Alexa Fluor® 594 goat anti-rabbit IgG (H+L), used at a 1/500 dilution.

    See Abreview

Anti-RFP antibody (ab62341) 使用論文

This product has been referenced in:
  • Szymanska E  et al. Impaired dynamin 2 function leads to increased AP-1 transcriptional activity through the JNK/c-Jun pathway. Cell Signal 28:160-71 (2016). Read more (PubMed: 26475677) »
  • Davey CF  et al. PCP Signaling between Migrating Neurons and their Planar-Polarized Neuroepithelial Environment Controls Filopodial Dynamics and Directional Migration. PLoS Genet 12:e1005934 (2016). IHC - Wholemount . Read more (PubMed: 26990447) »

See all 50 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Colon)
Specification Colon
Permeabilization Yes - Triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 18 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (Mouse Epiblast stem cells expressing Dsred)
Specification Mouse Epiblast stem cells expressing Dsred
Permeabilization Yes - PBS / 0.5% v/v Triton X-100 (Sigma)
Fixative Paraformaldehyde
Username

Miss. Yali Huang

Verified customer

投稿 Nov 29 2013

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Sample Rat Tissue sections (pituitary gland of mRFP1 gene transgenic rat)
Specification pituitary gland of mRFP1 gene transgenic rat
Permeabilization Yes - Triton-X
Fixative Paraformaldehyde
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投稿 Oct 30 2013

Application Western blot
Loading amount 10 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Sample Monkey Cell lysate - other (cos7 cell)
Specification cos7 cell
Treatment overexpress RFP construct
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 22°C
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投稿 Oct 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cell)
Specification Hela cell
Permeabilization No
Fixative Formaldehyde
Username

Ms. YA ZHOU

Verified customer

投稿 Sep 19 2013

Thank you for contacting us.

This product should recognize dsRed as well as RFP, but will not bind to GFP or coGFP. It has not been tested against mCherry however the immunogen only shares a 74% identity with the mCherry sequence (GenBank:...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (HT29 cells expressing DsRed protein)
Antigen retrieval step Enzymatic
Permeabilization Yes - Tween-20
Specification HT29 cells expressing DsRed protein
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde
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投稿 Sep 28 2015

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 56000 cells
Treatment Transfeceted for 24 hours
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Sep 23 2015

Yes, this product detects the DS red monomer. The WB in the image was run using dsRed monomer protein with a His-tag.

We do not have any in-house data or references showing reactivity with tdTomato, but a customer reported that the antibody did stain tdTomato in tissue of a transgenic mouse. Here is a link to the customer's review:

http://www.abcam.com/RFP-...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"