製品の概要

  • 製品名Anti-RFP antibody [25]
    RFP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [25] to RFP
  • 特異性ab65856 recognizes RFP and RFP-tagged fusion proteins.
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 免疫原

    Recombinant full length protein corresponding to RFP.

  • ポジティブ・コントロール
    • Recombinant RFP protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Proclin 300
    Constituents: Sodium chloride, Citrate, Tris, pH 7.0
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名25
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 22278251

ターゲット情報

  • 関連性Red fluorescent protein cloned from Entacmaea quadricolor eqFP611 (Uniprot IDQ8ISF8)is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).
  • 別名
    • GFP like chromoprotein antibody
    • red fluorescent protein antibody
    • Red fluorescent protein eqFP611 antibody
    • RFP antibody

Anti-RFP antibody [25] 画像

  • Anti-RFP antibody [25] (ab65856) at 1/1000 dilution + African Green Monkey Cell lysate - whole cell (overexpress pcDNA-mCherry) at 30 µg

    Secondary
    Sheep Mouse IgG, HRP-Linked F(ab')2 Fragment at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa


    Exposure time : 1 minute

    See Abreview

  • Anti-RFP antibody [25] (ab65856) at 1/1000 dilution + recombinant RFP protein

    Predicted band size : 26 kDa
    Observed band size : 26 kDa

Anti-RFP antibody [25] (ab65856) 使用論文

This product has been referenced in:
  • McWilliams TG  et al. mito-QC illuminates mitophagy and mitochondrial architecture in vivo. J Cell Biol 214:333-45 (2016). Read more (PubMed: 27458135) »
  • Hoon M  et al. Neurotransmission plays contrasting roles in the maturation of inhibitory synapses on axons and dendrites of retinal bipolar cells. Proc Natl Acad Sci U S A 112:12840-5 (2015). IHC ; Mouse . Read more (PubMed: 26420868) »

See all 8 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Rat Cultured Cells (Hippocampal neurons - primary culture)
Permeabilization No
Specification Hippocampal neurons - primary culture
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
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投稿 Apr 19 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample Human Tissue sections (HT29 cells expressing DsRed protein)
Specification HT29 cells expressing DsRed protein
Permeabilization Yes - Tween 20
Fixative Formaldehyde
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投稿 Mar 13 2015

We currently have no data for reactivity of anti-RFP ab65856 with DsRed-Express, native or denatured.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (overexpress pcDNA-mCherry)
Loading amount 30 µg
Specification overexpress pcDNA-mCherry
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Feb 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"