製品の概要
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製品名Anti-RFC2 antibody
RFC2 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to RFC2
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由来種Goat
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特異性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
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アプリケーション適用あり: WB, ICC/IFmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide (Human) - which represented a portion of human Replication Factor C 2 40 kDa subunit encoded within exon 1.
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ポジティブ･コントロール
- HeLa cell nuclear extract.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab3615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). | |
| ICC/IF | 1/1000. |
ターゲット情報
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機能The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
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関連疾患Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
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配列類似性Belongs to the activator 1 small subunits family.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 5982 Human
- Omim: 600404 Human
- SwissProt: P35250 Human
- Unigene: 647062 Human
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別名
- A1 40 kDa subunit antibody
- A1 antibody
- Activator 1 40 kDa subunit antibody
see all
画像
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Western blot - Anti-RFC2 antibody (ab3615)Sample: 50
µ g Hela cell nuclear extractanti-RFC 2 (exon 1) (ab3615) used at 1:1000 dilution
Sample: 50
µg Hela cell nuclear extract anti-RFC 2 (exon 1) (ab3615) used at 1:1000 dilution
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Immunocytochemistry/ Immunofluorescence - Anti-RFC2 antibody (ab3615)This image is courtesy of an Abreview submitted by Dr Kirk McManusab3615 (1/1000) staining RFC2 in assynchronous HeLa cells by ICC/IF, using a secondary Donkey anti-goat antibody (1/200) conjugated to Alexa Fluor® 488 (green). Cells were counterstained with DAPI (red) in order to show the nucleus.
参考文献
This product has been referenced in:
- Kawasoe Y et al. MutSa maintains the mismatch repair capability by inhibiting PCNA unloading. Elife 5:N/A (2016). Read more (PubMed: 27402201) »
- Nicolas A et al. Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments. J Virol 84:8871-87 (2010). IF . Read more (PubMed: 20573815) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"