製品の概要

  • 製品名Anti-RFC2 antibody
    RFC2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to RFC2
  • 特異性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Replication Factor C 2 40 kDa subunit encoded within exon 1.

  • ポジティブ・コントロール
    • HeLa cell nuclear extract.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報ICC/IF: 1/1000 (please see abreview).
    WB: 1/500 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.
    • 関連疾患Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
    • 配列類似性Belongs to the activator 1 small subunits family.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A1 40 kDa subunit antibody
      • A1 antibody
      • Activator 1 40 kDa subunit antibody
      • Activator 1 antibody
      • Activator 1 subunit 2 antibody
      • MGC3665 antibody
      • OTTHUMP00000160645 antibody
      • OTTHUMP00000160646 antibody
      • Replication factor C (activator 1) 2 40kDa antibody
      • Replication factor C (activator 1) 2 antibody
      • Replication factor C 2 antibody
      • Replication factor C 40 kDa subunit antibody
      • Replication factor C subunit 2 antibody
      • RF C 40 kDa subunit antibody
      • RF-C 40 kDa subunit antibody
      • RFC 2 antibody
      • RFC 40 antibody
      • RFC 40 kDa subunit antibody
      • RFC2 antibody
      • RFC2_HUMAN antibody
      • RFC40 antibody
      see all

    Anti-RFC2 antibody 画像



    • Predicted band size : 40 kDa


      Sample: 50µg Hela cell nuclear extract

      anti-RFC 2 (exon 1) (ab3615) used at 1:1000 dilution

    • ab3615 (1/1000) staining RFC2 in assynchronous HeLa cells by ICC/IF, using a secondary Donkey anti-goat antibody (1/200) conjugated to Alexa Fluor® 488 (green). Cells were counterstained with DAPI (red) in order to show the nucleus.

      See Abreview

    Anti-RFC2 antibody (ab3615) 使用論文

    ab3615 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Nov 02 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"