製品の概要

  • 製品名Anti-Rex1 antibody
    Rex1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Rex1
  • アプリケーション適用あり: IHC-P, ICC/IF, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab50828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50.
ICC/IF 1/50.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Involved in self-renewal property of ES cells (By similarity). May be involved in transcriptional regulation.
  • 組織特異性Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines.
  • 配列類似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hREX-1 antibody
    • Reduced Expression 1 Protein antibody
    • Reduced expression protein 1 antibody
    • Rex-1 antibody
    • REX1 antibody
    • REX1 transcription factor antibody
    • Zfp-42 antibody
    • Zfp42 antibody
    • ZFP42 zinc finger protein antibody
    • ZFP42_HUMAN antibody
    • Zinc finger protein 42 homolog antibody
    • Zinc finger protein 754 antibody
    • ZNF754 antibody
    see all

Anti-Rex1 antibody 画像

  • Anti-Rex1 antibody (ab50828) at 0.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1: 50,000 - 100,000)

    Predicted band size : 35 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence on Human Embryonic Stem Cells using Rex1 antibody (ab50828). Left hand image represents REX1 staining detected with AlexaFluor 633 secondary antibody (red). Right hand image represents REX1 staining (red) overlayed by nuclear DAPI staining (blue). See abreview for more details.
  • ab50828 staining Rex1 in human tissue sections (ESC-derived embryoid bodies) by immunohistochemistry (paraformaldehyde fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 8% Serum treatment in automated Bond Max system for 15 minutes. Samples were incubated with primary antibody (1/50) for 15 minutes. An HRP-conjugated goat anti-rabbit polyclonal (1/200) was used as secondary antibody.

    See Abreview

  • Immunofluorescence analysis of Human umbilical cord blood-derived mesenchymal stem cells, staining Rex1 with ab50828.

    Cells were fixed with paraformaldehyde for 20 minutes at room temperature, and incubated with 10% normal goat serum overnight at 4°C. Samples were incubated overnight at 4°C with primary antibody diluted in blocking solution followed by an AlexaFluor® anti-rabbit IgG secondary antibody for 1 hour.

Anti-Rex1 antibody (ab50828) 使用論文

This product has been referenced in:
  • Makhlouf M  et al. A prominent and conserved role for YY1 in Xist transcriptional activation. Nat Commun 5:4878 (2014). Read more (PubMed: 25209548) »
  • Fan A  et al. Effects of TET1 knockdown on gene expression and DNA methylation in porcine induced pluripotent stem cells. Reproduction 146:569-79 (2013). Read more (PubMed: 24051058) »

See all 6 Publications for this product

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We have not yet tested ab50828 in flow cytometry as yet and therefore do not know how well ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human ES cell)
Loading amount 10 µg
Specification human ES cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (hESC-derived embryoid bodies)
Specification hESC-derived embryoid bodies
Fixative Paraformaldehyde
Antigen retrieval step Other
Permeabilization No
Blocking step Serum provided by automated Bondmax System as blocking agent for 15 minute(s) · Concentration: 8%
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投稿 Apr 21 2010

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonal carcinoma cell lines)
Loading amount 20 µg
Specification mouse embryonal carcinoma cell lines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Sep 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human Embryonic Stem Cells)
Specification Human Embryonic Stem Cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 for 15min at r.t.
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 21°C
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投稿 Jul 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"